Fluorescence-Based Protein Footprinting Using Histidine-Tagged Protein
We describe a procedure for protein footprinting to identify the region(s) of a protein that interacts with a ligand. The method utilized the affinity of a stretch of histidine residues cloned into the protein to metalchelated resin. After limited protease digestion, the histidine-tagged end fragmen...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98241st04 |