Engineered human meniscus' matrix-forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions.
Low oxygen and mechanical loading may play roles in regulating the fibrocartilaginous phenotype of the human inner meniscus, but their combination in engineered tissues remains unstudied. Here, we investigated how continuous low oxygen ("hypoxia") combined with dynamic compression would af...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0248292 |
| _version_ | 1819038050397716480 |
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| author | Alexander R A Szojka Colleen N Moore Yan Liang Stephen H J Andrews Melanie Kunze Aillette Mulet-Sierra Nadr M Jomha Adetola B Adesida |
| author_facet | Alexander R A Szojka Colleen N Moore Yan Liang Stephen H J Andrews Melanie Kunze Aillette Mulet-Sierra Nadr M Jomha Adetola B Adesida |
| author_sort | Alexander R A Szojka |
| collection | DOAJ |
| description | Low oxygen and mechanical loading may play roles in regulating the fibrocartilaginous phenotype of the human inner meniscus, but their combination in engineered tissues remains unstudied. Here, we investigated how continuous low oxygen ("hypoxia") combined with dynamic compression would affect the fibrocartilaginous "inner meniscus-like" matrix-forming phenotype of human meniscus fibrochondrocytes (MFCs) in a porous type I collagen scaffold. Freshly-seeded MFC scaffolds were cultured for 4 weeks in either 3 or 20% O2 or pre-cultured for 2 weeks in 3% O2 and then dynamically compressed for 2 weeks (10% strain, 1 Hz, 1 h/day, 5 days/week), all with or without TGF-β3 supplementation. TGF-β3 supplementation was found necessary to induce matrix formation by MFCs in the collagen scaffold regardless of oxygen tension and application of the dynamic compression loading regime. Neither hypoxia under static culture nor hypoxia combined with dynamic compression had significant effects on expression of specific protein and mRNA markers for the fibrocartilaginous matrix-forming phenotype. Mechanical properties significantly increased over the two-week loading period but were not different between static and dynamic-loaded tissues after the loading period. These findings indicate that 3% O2 applied immediately after scaffold seeding and dynamic compression to 10% strain do not affect the fibrocartilaginous matrix-forming phenotype of human MFCs in this type I collagen scaffold. It is possible that a delayed hypoxia treatment and an optimized pre-culture period and loading regime combination would have led to different outcomes. |
| first_indexed | 2024-12-21T08:31:09Z |
| format | Article |
| id | doaj.art-a081f5cedfb645bab934725ca31e1033 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T08:31:09Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a081f5cedfb645bab934725ca31e10332022-12-21T19:10:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01163e024829210.1371/journal.pone.0248292Engineered human meniscus' matrix-forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions.Alexander R A SzojkaColleen N MooreYan LiangStephen H J AndrewsMelanie KunzeAillette Mulet-SierraNadr M JomhaAdetola B AdesidaLow oxygen and mechanical loading may play roles in regulating the fibrocartilaginous phenotype of the human inner meniscus, but their combination in engineered tissues remains unstudied. Here, we investigated how continuous low oxygen ("hypoxia") combined with dynamic compression would affect the fibrocartilaginous "inner meniscus-like" matrix-forming phenotype of human meniscus fibrochondrocytes (MFCs) in a porous type I collagen scaffold. Freshly-seeded MFC scaffolds were cultured for 4 weeks in either 3 or 20% O2 or pre-cultured for 2 weeks in 3% O2 and then dynamically compressed for 2 weeks (10% strain, 1 Hz, 1 h/day, 5 days/week), all with or without TGF-β3 supplementation. TGF-β3 supplementation was found necessary to induce matrix formation by MFCs in the collagen scaffold regardless of oxygen tension and application of the dynamic compression loading regime. Neither hypoxia under static culture nor hypoxia combined with dynamic compression had significant effects on expression of specific protein and mRNA markers for the fibrocartilaginous matrix-forming phenotype. Mechanical properties significantly increased over the two-week loading period but were not different between static and dynamic-loaded tissues after the loading period. These findings indicate that 3% O2 applied immediately after scaffold seeding and dynamic compression to 10% strain do not affect the fibrocartilaginous matrix-forming phenotype of human MFCs in this type I collagen scaffold. It is possible that a delayed hypoxia treatment and an optimized pre-culture period and loading regime combination would have led to different outcomes.https://doi.org/10.1371/journal.pone.0248292 |
| spellingShingle | Alexander R A Szojka Colleen N Moore Yan Liang Stephen H J Andrews Melanie Kunze Aillette Mulet-Sierra Nadr M Jomha Adetola B Adesida Engineered human meniscus' matrix-forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions. PLoS ONE |
| title | Engineered human meniscus' matrix-forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions. |
| title_full | Engineered human meniscus' matrix-forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions. |
| title_fullStr | Engineered human meniscus' matrix-forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions. |
| title_full_unstemmed | Engineered human meniscus' matrix-forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions. |
| title_short | Engineered human meniscus' matrix-forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions. |
| title_sort | engineered human meniscus matrix forming phenotype is unaffected by low strain dynamic compression under hypoxic conditions |
| url | https://doi.org/10.1371/journal.pone.0248292 |
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