Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia
Chronic lymphocytic leukemia (CLL) presents a varying incidence of karyotypic abnormalities whose detection is complicated by difficulties in obtaining mitosis for analysis in this type of mature lymphocyte disorder. Since the introduction of molecular cytogenetics (FISH = fluorescent in situ hybrid...
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Sociedade Brasileira de Genética
2000-09-01
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| Series: | Genetics and Molecular Biology |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300005 |
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| author | Maria de Lourdes L.F. Chauffaille Eliana Azevedo Marques Jose Salvador Rodrigues de Oliveira Maria Madalena Rodrigues Maria Stella Figueiredo Maura Romeo Mihoko Yamamoto José Kerbauy |
| author_facet | Maria de Lourdes L.F. Chauffaille Eliana Azevedo Marques Jose Salvador Rodrigues de Oliveira Maria Madalena Rodrigues Maria Stella Figueiredo Maura Romeo Mihoko Yamamoto José Kerbauy |
| author_sort | Maria de Lourdes L.F. Chauffaille |
| collection | DOAJ |
| description | Chronic lymphocytic leukemia (CLL) presents a varying incidence of karyotypic abnormalities whose detection is complicated by difficulties in obtaining mitosis for analysis in this type of mature lymphocyte disorder. Since the introduction of molecular cytogenetics (FISH = fluorescent in situ hybridization), applying centromeric probes for chromosome 12 has made it possible to detect a higher percentage of trisomy 12 cases. The objective of the present study was to detect trisomy 12 by FISH (alpha satellite probe) in 13 patients with CLL whose karyotypes by G-banding were either normal or inadequate. Using this method trisomy 12 was detected in three patients in a percentage of positive cells varying from 55.5% to 79%, showing that FISH is a sensitive and highly specific method for trisomy detection and should be routinely performed when the karyotype is normal.<br>A leucemia linfocítica crônica (CLL) apresenta incidência variável de anomalias de cariótipo devido às dificuldades em se obter mitose para análise. Desde a introdução da citogenética molecular (FISH = hibridação in situ por fluorescência) usando sonda centromérica para o cromossomo 12 foi possível detectar uma maior porcentagem de casos com trissomia 12. O objetivo deste trabalho foi de detectar trissomia 12 por FISH (sonda alfa satélite) em 13 pacientes com CLL cujos cariótipos por banda G haviam sido normais ou sem resultado. Três pacientes apresentaram trissomia 12 por este método com uma porcentagem de células trissômicas variando de 55,5 a 79%, demonstrando que a FISH é um método sensível e altamente específico para detecção de trissomia 12. |
| first_indexed | 2024-12-12T01:57:46Z |
| format | Article |
| id | doaj.art-a0902689ddf247ccbcc4ff987b0fcf0c |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-12T01:57:46Z |
| publishDate | 2000-09-01 |
| publisher | Sociedade Brasileira de Genética |
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| series | Genetics and Molecular Biology |
| spelling | doaj.art-a0902689ddf247ccbcc4ff987b0fcf0c2022-12-22T00:42:17ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852000-09-0123353153310.1590/S1415-47572000000300005Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemiaMaria de Lourdes L.F. ChauffailleEliana Azevedo MarquesJose Salvador Rodrigues de OliveiraMaria Madalena RodriguesMaria Stella FigueiredoMaura RomeoMihoko YamamotoJosé KerbauyChronic lymphocytic leukemia (CLL) presents a varying incidence of karyotypic abnormalities whose detection is complicated by difficulties in obtaining mitosis for analysis in this type of mature lymphocyte disorder. Since the introduction of molecular cytogenetics (FISH = fluorescent in situ hybridization), applying centromeric probes for chromosome 12 has made it possible to detect a higher percentage of trisomy 12 cases. The objective of the present study was to detect trisomy 12 by FISH (alpha satellite probe) in 13 patients with CLL whose karyotypes by G-banding were either normal or inadequate. Using this method trisomy 12 was detected in three patients in a percentage of positive cells varying from 55.5% to 79%, showing that FISH is a sensitive and highly specific method for trisomy detection and should be routinely performed when the karyotype is normal.<br>A leucemia linfocítica crônica (CLL) apresenta incidência variável de anomalias de cariótipo devido às dificuldades em se obter mitose para análise. Desde a introdução da citogenética molecular (FISH = hibridação in situ por fluorescência) usando sonda centromérica para o cromossomo 12 foi possível detectar uma maior porcentagem de casos com trissomia 12. O objetivo deste trabalho foi de detectar trissomia 12 por FISH (sonda alfa satélite) em 13 pacientes com CLL cujos cariótipos por banda G haviam sido normais ou sem resultado. Três pacientes apresentaram trissomia 12 por este método com uma porcentagem de células trissômicas variando de 55,5 a 79%, demonstrando que a FISH é um método sensível e altamente específico para detecção de trissomia 12.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300005 |
| spellingShingle | Maria de Lourdes L.F. Chauffaille Eliana Azevedo Marques Jose Salvador Rodrigues de Oliveira Maria Madalena Rodrigues Maria Stella Figueiredo Maura Romeo Mihoko Yamamoto José Kerbauy Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia Genetics and Molecular Biology |
| title | Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia |
| title_full | Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia |
| title_fullStr | Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia |
| title_full_unstemmed | Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia |
| title_short | Detection of trisomy 12 by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia |
| title_sort | detection of trisomy 12 by fluorescent in situ hybridization fish in chronic lymphocytic leukemia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300005 |
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