Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas
The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. H...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-02-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671115003732 |
| _version_ | 1819181316577427456 |
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| author | Johannes Riegler Antje Ebert Xulei Qin Qi Shen Mouer Wang Mohamed Ameen Kazuki Kodo Sang-Ging Ong Won Hee Lee Grace Lee Evgenios Neofytou Joseph D. Gold Andrew J. Connolly Joseph C. Wu |
| author_facet | Johannes Riegler Antje Ebert Xulei Qin Qi Shen Mouer Wang Mohamed Ameen Kazuki Kodo Sang-Ging Ong Won Hee Lee Grace Lee Evgenios Neofytou Joseph D. Gold Andrew J. Connolly Joseph C. Wu |
| author_sort | Johannes Riegler |
| collection | DOAJ |
| description | The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm3. A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm3 and with a sensitivity of more than 87%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking. |
| first_indexed | 2024-12-22T22:28:18Z |
| format | Article |
| id | doaj.art-a0b756cf3908414a863e8f913bab1d55 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-22T22:28:18Z |
| publishDate | 2016-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-a0b756cf3908414a863e8f913bab1d552022-12-21T18:10:29ZengElsevierStem Cell Reports2213-67112016-02-016217618710.1016/j.stemcr.2015.12.008Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived TeratomasJohannes Riegler0Antje Ebert1Xulei Qin2Qi Shen3Mouer Wang4Mohamed Ameen5Kazuki Kodo6Sang-Ging Ong7Won Hee Lee8Grace Lee9Evgenios Neofytou10Joseph D. Gold11Andrew J. Connolly12Joseph C. Wu13Stanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USADepartment of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Lorry Lokey Stem Cell Research Building, 265 Campus Drive, Stanford, CA 94305, USAThe use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm3. A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm3 and with a sensitivity of more than 87%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking.http://www.sciencedirect.com/science/article/pii/S2213671115003732magnetic resonance imagingultrasound imagingserum biomarkermicroRNA biomarkerpluripotent stem cellstumorigenicity |
| spellingShingle | Johannes Riegler Antje Ebert Xulei Qin Qi Shen Mouer Wang Mohamed Ameen Kazuki Kodo Sang-Ging Ong Won Hee Lee Grace Lee Evgenios Neofytou Joseph D. Gold Andrew J. Connolly Joseph C. Wu Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas Stem Cell Reports magnetic resonance imaging ultrasound imaging serum biomarker microRNA biomarker pluripotent stem cells tumorigenicity |
| title | Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas |
| title_full | Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas |
| title_fullStr | Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas |
| title_full_unstemmed | Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas |
| title_short | Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas |
| title_sort | comparison of magnetic resonance imaging and serum biomarkers for detection of human pluripotent stem cell derived teratomas |
| topic | magnetic resonance imaging ultrasound imaging serum biomarker microRNA biomarker pluripotent stem cells tumorigenicity |
| url | http://www.sciencedirect.com/science/article/pii/S2213671115003732 |
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