Adapting a conventional pcr assay for Toxoplasma gondii detection to real-time quantitative pcr including a competitive internal control

Adapting a conventional pcr assay for Toxoplasma gondii detection to real-time quantitative pcr including a competitive internal control

We have developed a quantitative PCR assay (LightCycler®) using the pair of primers JW58 and JW59 for the detection of the 35- fold repeated B1 gene of Toxoplasma gondii. This real-time PCR, using fluorescence resonance energy transfert (FRET) hybridization probes, allows the quantification of T. go...

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Bibliographic Details
Main Authors: Brenier-Pinchart M.P., Morand-Bui V., Fricker-Hidalgo H., Equy V., Marlu R., Pelloux H.
Format: Article
Language:English
Published: EDP Sciences 2007-06-01
Series:Parasite
Subjects:
Toxoplasma gondii
LightCycler®
real-time PCR
B1 gene
internal amplification control
Online Access:http://dx.doi.org/10.1051/parasite/2007142149

Internet

http://dx.doi.org/10.1051/parasite/2007142149

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