Adapting a conventional pcr assay for Toxoplasma gondii detection to real-time quantitative pcr including a competitive internal control
We have developed a quantitative PCR assay (LightCycler®) using the pair of primers JW58 and JW59 for the detection of the 35- fold repeated B1 gene of Toxoplasma gondii. This real-time PCR, using fluorescence resonance energy transfert (FRET) hybridization probes, allows the quantification of T. go...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2007-06-01
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| Series: | Parasite |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1051/parasite/2007142149 |
| Summary: | We have developed a quantitative PCR assay (LightCycler®) using the pair of primers JW58 and JW59 for the detection of the 35- fold repeated B1 gene of Toxoplasma gondii. This real-time PCR, using fluorescence resonance energy transfert (FRET) hybridization probes, allows the quantification of T. gondii with several technical requirements not previously described: i) an internal amplification control (co-amplified in a single tube with the same primers), ii) Uracil-N-Glycosylase and iii) a standard curve corresponding to a serial dilution from a calibrated suspension of T. gondii ranging from 40 to 4.106 parasites in one ml of amniotic fluid (1 to 105 T. gondii/PCR). In artificial samples, one parasite could be detected if at least three reactions were performed. |
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| ISSN: | 1252-607X 1776-1042 |