One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from <i>Streptomyces cacaoi</i> CA-170360
The strain <i>Streptomyces cacaoi</i> CA-170360 produces the cyclic pentapeptides pentaminomycins A–H and BE-18257 A–C, two families of cyclopeptides synthesized by two non-ribosomal peptide synthetases encoded in tandem within the same biosynthetic gene cluster. In this work, we have cl...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/1/135 |
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| author | Fernando Román-Hurtado Marina Sánchez-Hidalgo Jesús Martín Francisco Javier Ortiz-López Daniel Carretero-Molina Fernando Reyes Olga Genilloud |
| author_facet | Fernando Román-Hurtado Marina Sánchez-Hidalgo Jesús Martín Francisco Javier Ortiz-López Daniel Carretero-Molina Fernando Reyes Olga Genilloud |
| author_sort | Fernando Román-Hurtado |
| collection | DOAJ |
| description | The strain <i>Streptomyces cacaoi</i> CA-170360 produces the cyclic pentapeptides pentaminomycins A–H and BE-18257 A–C, two families of cyclopeptides synthesized by two non-ribosomal peptide synthetases encoded in tandem within the same biosynthetic gene cluster. In this work, we have cloned and confirmed the heterologous expression of this biosynthetic gene cluster, demonstrating that each of the non-ribosomal peptide synthetases present in the cluster is involved in the biosynthesis of each group of cyclopeptides. In addition, we discuss the involvement of a stand-alone enzyme belonging to the Penicillin Binding Protein family in the release and macrocyclization of the peptides. |
| first_indexed | 2024-03-09T05:32:30Z |
| format | Article |
| id | doaj.art-a0c1332f71f641eeb62929fc32a21554 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T05:32:30Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a0c1332f71f641eeb62929fc32a215542023-12-03T12:31:42ZengMDPI AGMicroorganisms2076-26072021-01-019113510.3390/microorganisms9010135One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from <i>Streptomyces cacaoi</i> CA-170360Fernando Román-Hurtado0Marina Sánchez-Hidalgo1Jesús Martín2Francisco Javier Ortiz-López3Daniel Carretero-Molina4Fernando Reyes5Olga Genilloud6Fundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, SpainFundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, SpainThe strain <i>Streptomyces cacaoi</i> CA-170360 produces the cyclic pentapeptides pentaminomycins A–H and BE-18257 A–C, two families of cyclopeptides synthesized by two non-ribosomal peptide synthetases encoded in tandem within the same biosynthetic gene cluster. In this work, we have cloned and confirmed the heterologous expression of this biosynthetic gene cluster, demonstrating that each of the non-ribosomal peptide synthetases present in the cluster is involved in the biosynthesis of each group of cyclopeptides. In addition, we discuss the involvement of a stand-alone enzyme belonging to the Penicillin Binding Protein family in the release and macrocyclization of the peptides.https://www.mdpi.com/2076-2607/9/1/135pentaminomycinBE-18257heterologous expressionCas9-assisted targeting of chromosome segments (CATCH) cloningnon-ribosomal peptide synthetases (NRPS)secondary metabolites |
| spellingShingle | Fernando Román-Hurtado Marina Sánchez-Hidalgo Jesús Martín Francisco Javier Ortiz-López Daniel Carretero-Molina Fernando Reyes Olga Genilloud One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from <i>Streptomyces cacaoi</i> CA-170360 Microorganisms pentaminomycin BE-18257 heterologous expression Cas9-assisted targeting of chromosome segments (CATCH) cloning non-ribosomal peptide synthetases (NRPS) secondary metabolites |
| title | One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from <i>Streptomyces cacaoi</i> CA-170360 |
| title_full | One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from <i>Streptomyces cacaoi</i> CA-170360 |
| title_fullStr | One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from <i>Streptomyces cacaoi</i> CA-170360 |
| title_full_unstemmed | One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from <i>Streptomyces cacaoi</i> CA-170360 |
| title_short | One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from <i>Streptomyces cacaoi</i> CA-170360 |
| title_sort | one pathway two cyclic non ribosomal pentapeptides heterologous expression of be 18257 antibiotics and pentaminomycins from i streptomyces cacaoi i ca 170360 |
| topic | pentaminomycin BE-18257 heterologous expression Cas9-assisted targeting of chromosome segments (CATCH) cloning non-ribosomal peptide synthetases (NRPS) secondary metabolites |
| url | https://www.mdpi.com/2076-2607/9/1/135 |
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