Microbial Spoilage, Actions of Preservatives and Phytochemical <span style="font-size: 12.0pt; font-family: 'Times New Roman',serif">Screening of Mango (<i>Mangifera indica</i>) Seed Powder
The work was carried out to determine the organisms responsible for the microbial spoilage of kernels of Mangifera indica. A specialized kit was employed to confirm the Gram negative organisms present in the spoilt kernels of M. indica. The effects of chemical preservatives such as sodium benzoate,...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2015-03-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9470 |
| _version_ | 1818506140604956672 |
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| author | Musa Olusegun AREKEMASE Ganiyu OYEYIOLA Fathia Oluwatoyin SAAD Daniel Salem TERWASE |
| author_facet | Musa Olusegun AREKEMASE Ganiyu OYEYIOLA Fathia Oluwatoyin SAAD Daniel Salem TERWASE |
| author_sort | Musa Olusegun AREKEMASE |
| collection | DOAJ |
| description | The work was carried out to determine the organisms responsible for the microbial spoilage of kernels of Mangifera indica. A specialized kit was employed to confirm the Gram negative organisms present in the spoilt kernels of M. indica. The effects of chemical preservatives such as sodium benzoate, sodium acetate, citric acid and sodium chloride at different concentrations on the microbial counts and pH of mango seed powder stored at room temperature over a period of 12 weeks were studied. The mango seed kernel powder (MSK) was screened for phytochemicals. The bacteria isolated include: Bacillus subtilis, Staphylococcus aureus, Enterobacter clocae, Enterobacter asburiae and Stenotrophomonas maltophilia. The Gram negative organisms confirmed were Enterobacter clocae, Enterobacter asburiae and Stenotrophomonas maltophilia. The isolated fungus was Aspergillus niger. In the analysis of different chemical preservatives on mango seed powder, the most effective preservative was 3.0% sodium benzoate followed by 5% sodium acetate and 5% common salt. Citric acid was the least effective of all the preservatives used at equal concentrations. Sodium benzoate at 3% had the least bacterial count of 0.8 x 103 CFU/ml which was maintained from the 8th week to the last week of storage. Citric acid at 0.1% and 1.0% concentrations had bacterial counts of 3.50 x 103 CFU/ml and 2.0 x 103 CFU/ml respectively at the end of the 12 weeks of storage. The pH of the chemically preserved powdered kernels of M. indica from the 1st to the 12th week ranged from 2.70-6.01. The phytochemicals present in the mango seed powder included tannins, saponnins, polyphenol, alkaloids, flavonoids, cardiac glycosides and steroids. |
| first_indexed | 2024-12-10T22:00:29Z |
| format | Article |
| id | doaj.art-a0da0c00871441a591d0868ee862b338 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-10T22:00:29Z |
| publishDate | 2015-03-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-a0da0c00871441a591d0868ee862b3382022-12-22T01:31:54ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642015-03-017110211010.15835/nsb7194708124Microbial Spoilage, Actions of Preservatives and Phytochemical <span style="font-size: 12.0pt; font-family: 'Times New Roman',serif">Screening of Mango (<i>Mangifera indica</i>) Seed PowderMusa Olusegun AREKEMASE0Ganiyu OYEYIOLA1Fathia Oluwatoyin SAAD2Daniel Salem TERWASE3Department of Microbiology, University of Ilorin, P. M. B. 1515, Ilorin, Kwara StateDepartment of Microbiology, University of Ilorin, P. M. B. 1515, Ilorin, Kwara StateDepartment of Microbiology, University of Ilorin, P. M. B. 1515, Ilorin, Kwara StateDepartment of Biological Sciences, Microbiology Unit, University of Agriculture, Makurdi, P.M. B. 2327 Makurdi, Benue StateThe work was carried out to determine the organisms responsible for the microbial spoilage of kernels of Mangifera indica. A specialized kit was employed to confirm the Gram negative organisms present in the spoilt kernels of M. indica. The effects of chemical preservatives such as sodium benzoate, sodium acetate, citric acid and sodium chloride at different concentrations on the microbial counts and pH of mango seed powder stored at room temperature over a period of 12 weeks were studied. The mango seed kernel powder (MSK) was screened for phytochemicals. The bacteria isolated include: Bacillus subtilis, Staphylococcus aureus, Enterobacter clocae, Enterobacter asburiae and Stenotrophomonas maltophilia. The Gram negative organisms confirmed were Enterobacter clocae, Enterobacter asburiae and Stenotrophomonas maltophilia. The isolated fungus was Aspergillus niger. In the analysis of different chemical preservatives on mango seed powder, the most effective preservative was 3.0% sodium benzoate followed by 5% sodium acetate and 5% common salt. Citric acid was the least effective of all the preservatives used at equal concentrations. Sodium benzoate at 3% had the least bacterial count of 0.8 x 103 CFU/ml which was maintained from the 8th week to the last week of storage. Citric acid at 0.1% and 1.0% concentrations had bacterial counts of 3.50 x 103 CFU/ml and 2.0 x 103 CFU/ml respectively at the end of the 12 weeks of storage. The pH of the chemically preserved powdered kernels of M. indica from the 1st to the 12th week ranged from 2.70-6.01. The phytochemicals present in the mango seed powder included tannins, saponnins, polyphenol, alkaloids, flavonoids, cardiac glycosides and steroids.http://www.notulaebiologicae.ro/index.php/nsb/article/view/9470 |
| spellingShingle | Musa Olusegun AREKEMASE Ganiyu OYEYIOLA Fathia Oluwatoyin SAAD Daniel Salem TERWASE Microbial Spoilage, Actions of Preservatives and Phytochemical <span style="font-size: 12.0pt; font-family: 'Times New Roman',serif">Screening of Mango (<i>Mangifera indica</i>) Seed Powder Notulae Scientia Biologicae |
| title | Microbial Spoilage, Actions of Preservatives and Phytochemical <span style="font-size: 12.0pt; font-family: 'Times New Roman',serif">Screening of Mango (<i>Mangifera indica</i>) Seed Powder |
| title_full | Microbial Spoilage, Actions of Preservatives and Phytochemical <span style="font-size: 12.0pt; font-family: 'Times New Roman',serif">Screening of Mango (<i>Mangifera indica</i>) Seed Powder |
| title_fullStr | Microbial Spoilage, Actions of Preservatives and Phytochemical <span style="font-size: 12.0pt; font-family: 'Times New Roman',serif">Screening of Mango (<i>Mangifera indica</i>) Seed Powder |
| title_full_unstemmed | Microbial Spoilage, Actions of Preservatives and Phytochemical <span style="font-size: 12.0pt; font-family: 'Times New Roman',serif">Screening of Mango (<i>Mangifera indica</i>) Seed Powder |
| title_short | Microbial Spoilage, Actions of Preservatives and Phytochemical <span style="font-size: 12.0pt; font-family: 'Times New Roman',serif">Screening of Mango (<i>Mangifera indica</i>) Seed Powder |
| title_sort | microbial spoilage actions of preservatives and phytochemical lt span style font size 12 0pt font family times new roman serif gt screening of mango lt i gt mangifera indica lt i gt seed powder |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9470 |
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