Simultaneous Identification of Functional Antigen-Specific CD8<sup>+</sup> and CD4<sup>+</sup> Cells after In Vitro Expansion Using Elongated Peptides

Simultaneous Identification of Functional Antigen-Specific CD8<sup>+</sup> and CD4<sup>+</sup> Cells after In Vitro Expansion Using Elongated Peptides

Elongated peptides (EPs), containing possibly one or multiple epitope/s, are increasingly used for the screening of antigen-specific CD8<sup>+</sup> and CD4<sup>+</sup> cell responses. Here, we present an in vitro protocol that allows the amplification of antigen-specific cel...

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Bibliographic Details
Main Authors: Juliane Schuhmacher, Leon Kleemann, Jennifer Rebecca Richardson, Elisa Rusch, Hans-Georg Rammensee, Cécile Gouttefangeas
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Cells
Subjects:
T cells
immunomonitoring
elongated peptides
ELISpot
intracellular cytokine staining
Online Access:https://www.mdpi.com/2073-4409/11/21/3451

Internet

https://www.mdpi.com/2073-4409/11/21/3451

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