Stabilization of glucose oxidase with cyclodextrin-branched carboxymethylcellulose

Stabilization of glucose oxidase with cyclodextrin-branched carboxymethylcellulose

We present a methodology for improving some enzymatic characteristics of glucose oxidase (GOx) (EC 1.1.3.4). The enzyme was chemically modified with a ß-cyclodextrin-branched carboxymethylcellulose polymer (CMC-CD), using carbodiimide as coupling agent. The obtained neoglycoenzyme had 0.78 mol of po...

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Bibliographic Details
Main Authors: Madyu Matos, Benjamin K Simpson, Héctor L Ramírez, Roberto Cao, Juan J Torres-Labandeira, Karel Hernández
Format: Article
Language:English
Published: Elfos Scientiae
Series:Biotecnología Aplicada
Subjects:
carboxymethylcellulose
cyclodextrin
enzyme stability
glucose oxidase
Online Access:http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522012000100005&lng=en&tlng=en

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http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522012000100005&lng=en&tlng=en

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