Development of organophosphate hydrolase activity in a bacterial homolog of human cholinesterase

Development of organophosphate hydrolase activity in a bacterial homolog of human cholinesterase

We applied a combination of rational design and directed evolution (DE) to Bacillus subtilis p-nitrobenzyl esterase (pNBE) with the goal of enhancing organophosphorus acid anhydride hydrolase (OPAAH) activity. DE started with a designed variant, pNBE A107H, carrying a histidine homologous with huma...

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Bibliographic Details
Main Authors: Patricia Marie Legler, Susanne eBoisvert, Jaimee R Compton, Charles B. Millard
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-07-01
Series:Frontiers in Chemistry
Subjects:
Directed Evolution
organophosphate
cholinesterase
catalytic bioscavenger
nerve agent
Online Access:http://journal.frontiersin.org/Journal/10.3389/fchem.2014.00046/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fchem.2014.00046/full

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