Comparative Use of Quantitative PCR (qPCR), Droplet Digital PCR (ddPCR), and Recombinase Polymerase Amplification (RPA) in the Detection of Shiga Toxin-Producing <i>E. coli</i> (STEC) in Environmental Samples

Comparative Use of Quantitative PCR (qPCR), Droplet Digital PCR (ddPCR), and Recombinase Polymerase Amplification (RPA) in the Detection of Shiga Toxin-Producing <i>E. coli</i> (STEC) in Environmental Samples

<i>E. coli</i> O157:H7 is a foodborne pathogen that constitutes a global threat to human health. However, the quantification of this pathogen in food and environmental samples may be problematic at the low cell numbers commonly encountered in environmental samples. In this study, we used...

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Bibliographic Details
Main Authors: Mark A. Ibekwe, Shelton E. Murinda, Stanley Park, Amarachukwu Obayiuwana, Marcia A. Murry, Gregory Schwartz, Trygve Lundquist
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Water
Subjects:
Shiga toxin-producing <i>E. coli</i>
high rate algae ponds
dairy lagoon effluent
quantitative PCR
droplet digital PCR
recombinase polymerase amplification
Online Access:https://www.mdpi.com/2073-4441/12/12/3507

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https://www.mdpi.com/2073-4441/12/12/3507

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