Generation of human induced pluripotent stem cells in defined, feeder-free conditions

Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately,...

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Bibliographic Details
Main Authors: Athena Kyrkou, Dimitris Stellas, Marika Syrrou, Apostolos Klinakis, Theodore Fotsis, Carol Murphy
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506116300381