Organoid Sample Preparation and Extraction for LC-MS Peptidomics

Organoid Sample Preparation and Extraction for LC-MS Peptidomics

Summary: This protocol describes the peptidomic analysis of organoid lysates, FACS-purified cell populations, and 2D culture secretions by liquid chromatography mass spectrometry (LC-MS). Currently, most peptides are quantified by ELISA, limiting the peptides that can be studied. However, an LC-MS-b...

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Bibliographic Details
Main Authors: Emily L. Miedzybrodzka, Rachel E. Foreman, Sam G. Galvin, Pierre Larraufie, Amy L. George, Deborah A. Goldspink, Frank Reimann, Fiona M. Gribble, Richard G. Kay
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Proteomics
Organoids
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301519

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http://www.sciencedirect.com/science/article/pii/S2666166720301519

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