Evaluation of a rapid molecular method for detection of Listeria monocytogenes directly from broth culture

The present study was carried out to know the lowest detection limit of Listeria monocytogenes by PCR. The quantification of organisms was done by CFU counting from ten fold serial dilution and PCR amplification of inlA gene fragment was performed from each dilution. The PCR could detect as low as 2...

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Bibliographic Details
Main Authors: R. A. Mathakiya, A. Roy, K. N. Nandasana, P. G. Koringa and C.G. Joshi
Format: Article
Language:English
Published: Veterinary World 2009-10-01
Series:Veterinary World
Subjects:
Online Access:http://www.scopemed.org/mnstemps/2/2-1273845115.pdf