Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp.
In order to biodegrade natural chitin, in this study, chitin was used as the sole carbon source, obtain chitin deacetylase strains which could produce chitosan from natural chitin by biological method. Results showed that, the strains were identified by color plate screening and enzyme activity resc...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-12-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022020120 |
| _version_ | 1811178466750496768 |
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| author | Xin MENG Guofeng AI Guotao JIANG Keyi XIE Aili JIANG |
| author_facet | Xin MENG Guofeng AI Guotao JIANG Keyi XIE Aili JIANG |
| author_sort | Xin MENG |
| collection | DOAJ |
| description | In order to biodegrade natural chitin, in this study, chitin was used as the sole carbon source, obtain chitin deacetylase strains which could produce chitosan from natural chitin by biological method. Results showed that, the strains were identified by color plate screening and enzyme activity rescreening, morphological characteristics and 16S rRNA sequence analysis. A bacterium X4 with higher activity of chitin deacetylase was obtained, and was identified as Lysinibacillus sp.. The fermentation culture of the strain showed that the activity of chitin deacetylase produced by X4 could reached 8.210 U/mL, and the deacetylation degree of chitin was 8.642%. The results was valuable for the green biological utilization of chitin. |
| first_indexed | 2024-04-11T06:18:48Z |
| format | Article |
| id | doaj.art-a15c1bb616b8470093444933edc66361 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-11T06:18:48Z |
| publishDate | 2022-12-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-a15c1bb616b8470093444933edc663612022-12-22T04:40:58ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-12-01432413914410.13386/j.issn1002-0306.20220201202022020120-24Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp.Xin MENG0Guofeng AI1Guotao JIANG2Keyi XIE3Aili JIANG4College of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaIn order to biodegrade natural chitin, in this study, chitin was used as the sole carbon source, obtain chitin deacetylase strains which could produce chitosan from natural chitin by biological method. Results showed that, the strains were identified by color plate screening and enzyme activity rescreening, morphological characteristics and 16S rRNA sequence analysis. A bacterium X4 with higher activity of chitin deacetylase was obtained, and was identified as Lysinibacillus sp.. The fermentation culture of the strain showed that the activity of chitin deacetylase produced by X4 could reached 8.210 U/mL, and the deacetylation degree of chitin was 8.642%. The results was valuable for the green biological utilization of chitin.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022020120chitin deacetylaselysinibacillus sp.enzyme activitydegree of deacetylationstrain identification |
| spellingShingle | Xin MENG Guofeng AI Guotao JIANG Keyi XIE Aili JIANG Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp. Shipin gongye ke-ji chitin deacetylase lysinibacillus sp. enzyme activity degree of deacetylation strain identification |
| title | Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp. |
| title_full | Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp. |
| title_fullStr | Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp. |
| title_full_unstemmed | Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp. |
| title_short | Screening and Enzymatic-producing Study of Chitin Deacetylase Producing Bacteria-Lysinibacillus sp. |
| title_sort | screening and enzymatic producing study of chitin deacetylase producing bacteria lysinibacillus sp |
| topic | chitin deacetylase lysinibacillus sp. enzyme activity degree of deacetylation strain identification |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022020120 |
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