Generation of a human embryonic stem cell reporter line, TMEM119-EGFP, for the visualisation of in vitro differentiated human microglia

Generation of a human embryonic stem cell reporter line, TMEM119-EGFP, for the visualisation of in vitro differentiated human microglia

Transmembrane protein 119 (TMEM119) is a recently identified microglia marker that is not expressed by other immune cells. Using CRISPR/Cas9 technology, we introduced enhanced green fluorescence protein (EGFP), into the H9 WA-09 human embryonic stem cell line, directly before the TMEM119 stop codon....

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Bibliographic Details
Main Authors: Charlotte R. Denman, Hoang-Dai Tran, Min-Kyoung Shin, Jisoo Ryu, Bernd Kuhn, Junghyun Jo
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506123002507

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http://www.sciencedirect.com/science/article/pii/S1873506123002507

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