In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool
Megakaryocytes (MKs) are relatively rare in bone marrow, comprising <0.05% of the nucleated cells, which makes direct isolation from human bone marrow impractical. As such, in vitro expansion of primary MKs from patient samples offers exciting fundamental and clinical opportunities. As most of th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-10-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2020.1817359 |
| _version_ | 1797684155341864960 |
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| author | Kirill R Butov Elena Y Osipova Nikita B Mikhalkin Natalia M Trubina Mikhail A Panteleev Kellie R Machlus |
| author_facet | Kirill R Butov Elena Y Osipova Nikita B Mikhalkin Natalia M Trubina Mikhail A Panteleev Kellie R Machlus |
| author_sort | Kirill R Butov |
| collection | DOAJ |
| description | Megakaryocytes (MKs) are relatively rare in bone marrow, comprising <0.05% of the nucleated cells, which makes direct isolation from human bone marrow impractical. As such, in vitro expansion of primary MKs from patient samples offers exciting fundamental and clinical opportunities. As most of the developed ex vivo methods require a substantial volume of biomaterial, they are not widely performed on young patients. Here we propose a simple, robust, and adapted method of primary human MK culture from 1 mL of bone marrow aspirate. Our technique uses a small volume of bone marrow per culture, uses straightforward isolation methods, and generates approximately 6 × 105 mature MKs per culture. The relative high cell purity and yield achieved by this technique, combined with efficient use of low volumes of bone marrow, make this approach suitable for diagnostic and basic research of human megakaryopoiesis. |
| first_indexed | 2024-03-12T00:26:26Z |
| format | Article |
| id | doaj.art-a19f418c183148269a5b3941b8fc771d |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:26:26Z |
| publishDate | 2021-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-a19f418c183148269a5b3941b8fc771d2023-09-15T10:38:08ZengTaylor & Francis GroupPlatelets0953-71041369-16352021-10-0132792893510.1080/09537104.2020.18173591817359In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic toolKirill R Butov0Elena Y Osipova1Nikita B Mikhalkin2Natalia M Trubina3Mikhail A Panteleev4Kellie R Machlus5Dmitry Rogachev National Medical Research Center of Pediatric HematologyDmitry Rogachev National Medical Research Center of Pediatric HematologyCenter for Theoretical Problems of Physicochemical PharmacologyDmitry Rogachev National Medical Research Center of Pediatric HematologyCenter for Theoretical Problems of Physicochemical PharmacologyHematology Department, Brigham and Women’s Hospital Division of Hematology and Harvard Medical School Department of Medicine, Boston, MA, USAMegakaryocytes (MKs) are relatively rare in bone marrow, comprising <0.05% of the nucleated cells, which makes direct isolation from human bone marrow impractical. As such, in vitro expansion of primary MKs from patient samples offers exciting fundamental and clinical opportunities. As most of the developed ex vivo methods require a substantial volume of biomaterial, they are not widely performed on young patients. Here we propose a simple, robust, and adapted method of primary human MK culture from 1 mL of bone marrow aspirate. Our technique uses a small volume of bone marrow per culture, uses straightforward isolation methods, and generates approximately 6 × 105 mature MKs per culture. The relative high cell purity and yield achieved by this technique, combined with efficient use of low volumes of bone marrow, make this approach suitable for diagnostic and basic research of human megakaryopoiesis.http://dx.doi.org/10.1080/09537104.2020.1817359bone marrowhumanmegakaryocyteproplatelet |
| spellingShingle | Kirill R Butov Elena Y Osipova Nikita B Mikhalkin Natalia M Trubina Mikhail A Panteleev Kellie R Machlus In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool Platelets bone marrow human megakaryocyte proplatelet |
| title | In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool |
| title_full | In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool |
| title_fullStr | In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool |
| title_full_unstemmed | In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool |
| title_short | In vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool |
| title_sort | in vitro megakaryocyte culture from human bone marrow aspirates as a research and diagnostic tool |
| topic | bone marrow human megakaryocyte proplatelet |
| url | http://dx.doi.org/10.1080/09537104.2020.1817359 |
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