Protocol to determine antibody affinity and concentration in complex solutions using microfluidic antibody affinity profiling

Summary: Conventional methods of measuring affinity are limited by artificial immobilization, large sample volumes, and homogeneous solutions. This protocol describes microfluidic antibody affinity profiling on complex human samples in solution to obtain a fingerprint reflecting both affinity and ac...

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Bibliographic Details
Main Authors: Marc Emmenegger, Roland Worth, Sebastian Fiedler, Sean R.A. Devenish, Tuomas P.J. Knowles, Adriano Aguzzi
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000539