Protocol for the isolation and culture of microglia, astrocytes, and neurons from the same mouse brain

Summary: Studying the intrinsic properties of microglia, astrocytes, and neurons is essential to our understanding of brain function. Here, we present a protocol to isolate and culture these neural cells from the same mouse brain. Using immunocapture magnetic beads, we describe steps for dissociatin...

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Bibliographic Details
Main Authors: Elvira P. Leites, Vanessa A. Morais
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007712