Blood handling and leukocyte isolation methods impact the global transcriptome of immune cells

Blood handling and leukocyte isolation methods impact the global transcriptome of immune cells

Abstract Background Transcriptional profiling with ultra-low input methods can yield valuable insights into disease, particularly when applied to the study of immune cells using RNA-sequencing. The advent of these methods has allowed for their use in profiling cells collected in clinical trials and...

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Bibliographic Details
Main Authors: Brittany A. Goods, Jacqueline M. Vahey, Arthur F. Steinschneider, Michael H. Askenase, Lauren Sansing, J. Christopher Love
Format: Article
Language:English
Published: BMC 2018-10-01
Series:BMC Immunology
Subjects:
Immune profiling
Peripheral blood mononuclear cells
Transcriptome
RNA-seq
Online Access:http://link.springer.com/article/10.1186/s12865-018-0268-6

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http://link.springer.com/article/10.1186/s12865-018-0268-6

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