Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein

Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while gly...

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Bibliographic Details
Main Authors: Puneet Jain, Takuya Aida, Masahiro Motosuke
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Micromachines
Subjects:
Online Access:https://www.mdpi.com/2072-666X/12/9/1109