On technique for counting of fish pyloric appendages

New technique for collecting, fixing and counting of fish pyloric appendages is described that does not use any chemical fixing reagent. It includes storing of chemically untreated deep-frozen samples during unlimited time, defrosting the samples, and heat treatment the samples in microwave oven dep...

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Bibliographic Details
Main Author: Anna E. Lapshina
Format: Article
Language:Russian
Published: Transactions of the Pacific Research Institute of Fisheries and Oceanography 2014-06-01
Series:Известия ТИНРО
Subjects:
Online Access:https://izvestiya.tinro-center.ru/jour/article/view/22
Description
Summary:New technique for collecting, fixing and counting of fish pyloric appendages is described that does not use any chemical fixing reagent. It includes storing of chemically untreated deep-frozen samples during unlimited time, defrosting the samples, and heat treatment the samples in microwave oven depending on weight of sample and power of the oven. The technique allows to avoid work with toxic reagents, as ethanol and formalin, and to lower the costs of investigation.
ISSN:1606-9919
2658-5510