Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix

Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix

Summary: This protocol describes the differentiation of human neural progenitor cells (hNPCs) in a microfluidic device containing a thin 3D matrix with two separate chambers, enabling a cleaner separation between axons and soma/bulk neurons. We have used this technique to study how mitochondria-asso...

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Bibliographic Details
Main Authors: Madhura S. Lotlikar, Marina B. Tarantino, Mehdi Jorfi, Dora M. Kovacs, Rudolph E. Tanzi, Raja Bhattacharyya
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Biotechnology and bioengineering
Cell Biology
Cell culture
Cell-based Assays
Neuroscience
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007346

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http://www.sciencedirect.com/science/article/pii/S2666166721007346

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