Scanning mutagenesis of the voltage-gated sodium channel NaV1.2 using base editing

Scanning mutagenesis of the voltage-gated sodium channel NaV1.2 using base editing

Summary: It is challenging to apply traditional mutational scanning to voltage-gated sodium channels (NaVs) and functionally annotate the large number of coding variants in these genes. Using a cytosine base editor and a pooled viability assay, we screen a library of 368 guide RNAs (gRNAs) tiling Na...

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Bibliographic Details
Main Authors: Juan Lorenzo B. Pablo, Savannah L. Cornett, Lei A. Wang, Sooyeon Jo, Tobias Brünger, Nikita Budnik, Mudra Hegde, Jean-Marc DeKeyser, Christopher H. Thompson, John G. Doench, Dennis Lal, Alfred L. George, Jr., Jen Q. Pan
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Cell Reports
Subjects:
CP: Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124723005740

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http://www.sciencedirect.com/science/article/pii/S2211124723005740

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