Improving Stability and Specificity of CRISPR/Cas9 System by Selective Modification of Guide RNAs with 2′-fluoro and Locked Nucleic Acid Nucleotides

Improving Stability and Specificity of CRISPR/Cas9 System by Selective Modification of Guide RNAs with 2′-fluoro and Locked Nucleic Acid Nucleotides

The genome editing approach using the components of the CRISPR/Cas system has found wide application in molecular biology, fundamental medicine and genetic engineering. A promising method is to increase the efficacy and specificity of CRISPR/Cas-based genome editing systems by modifying their compon...

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Bibliographic Details
Main Authors: Lubov Sakovina, Ivan Vokhtantsev, Mariya Vorobyeva, Pavel Vorobyev, Darya Novopashina
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:International Journal of Molecular Sciences
Subjects:
CRISPR
crRNA
tracrRNA
sgRNA
gene editing
2’-modification
Online Access:https://www.mdpi.com/1422-0067/23/21/13460

Internet

https://www.mdpi.com/1422-0067/23/21/13460

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