RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding

RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding

Abstract Progress in sample preparation for scRNA-seq is reported based on RevGel-seq, a reversible-hydrogel technology optimized for samples of fresh cells. Complexes of one cell paired with one barcoded bead are stabilized by a chemical linker and dispersed in a hydrogel in the liquid state. Upon...

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Bibliographic Details
Main Authors: Jun Komatsu, Alba Cico, Raya Poncin, Maël Le Bohec, Jörg Morf, Stanislav Lipin, Antoine Graindorge, Hélène Eckert, Azadeh Saffarian, Léa Cathaly, Frédéric Guérin, Sara Majello, Damien Ulveling, Anaïs Vayaboury, Nicolas Fernandez, Dilyana Dimitrova, Xavier Bussell, Yannick Fourne, Pierre Chaumat, Barbara André, Elodie Baldivia, Ulysse Godet, Mathieu Guinin, Vivien Moretto, Joy Ismail, Olivier Caille, Natacha Roblot, Carine Beaupère, Alexandrine Liboz, Ghislaine Guillemain, Bertrand Blondeau, Pierre Walrafen, Stuart Edelstein
Format: Article
Language:English
Published: Nature Portfolio 2023-03-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-31915-y

Internet

https://doi.org/10.1038/s41598-023-31915-y

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