Chemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in Turkey
Many species of the Allium genus, principally the cultivated forms, are widely used as vegetables, spices and natural therapeutics due to their beneficial health properties. This study aimed to identify the phenolic composition and biological activities of the bulb, stem and flower parts of Allium p...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2020-01-01
|
| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000013E.pdf |
| _version_ | 1818538355689783296 |
|---|---|
| author | Emir Ahmet Emir Ceren |
| author_facet | Emir Ahmet Emir Ceren |
| author_sort | Emir Ahmet |
| collection | DOAJ |
| description | Many species of the Allium genus, principally the cultivated forms, are widely used as vegetables, spices and natural therapeutics due to their beneficial health properties. This study aimed to identify the phenolic composition and biological activities of the bulb, stem and flower parts of Allium pallens L., collected from two different localities. A total of 28 phenolic compounds were investigated by LC-ESI-MS/MS, and gallic acid, 4-hydroxybenzoic acid, and benzoic acid were found to be the major phenolic compounds in the plants from both locations. Total phenolic- and flavonoid-content analyses of samples were carried out using spectrophotometry, and the stem extracts were found to be rich in phenolics. DPPH, ABTS, FRAP and CUPRAC assays were used to determine the antioxidant capacities of the extracts. A linear relation was observed between the phenolic contents of the extracts and their antioxidant activities, and the stem extracts of plants from both locations were found to have potent antioxidant capacity. The inhibitory activities of the extracts against acetylcholinesterase, butyrylcholinesterase and tyrosinase were determined using a 96-well microplate reader. The antibutyrylcholinesterase activity of the extracts was found to be the highest. The outcomes of these investigations were further explored, and the underlying structure of multivariate data was revealed using principal component analysis. This study presents the distribution of chemical constituents and biological activities of the different parts of A. pallens, and also contributes to further investigations of Allium species. |
| first_indexed | 2024-12-11T21:27:56Z |
| format | Article |
| id | doaj.art-a2911266d6914e3ea00bea64f7dc41b2 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-11T21:27:56Z |
| publishDate | 2020-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-a2911266d6914e3ea00bea64f7dc41b22022-12-22T00:50:17ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392020-01-0172219320110.2298/ABS200226013E0354-46642000013EChemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in TurkeyEmir Ahmet0Emir Ceren1Department of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir, TurkeyDepartment of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir, TurkeyMany species of the Allium genus, principally the cultivated forms, are widely used as vegetables, spices and natural therapeutics due to their beneficial health properties. This study aimed to identify the phenolic composition and biological activities of the bulb, stem and flower parts of Allium pallens L., collected from two different localities. A total of 28 phenolic compounds were investigated by LC-ESI-MS/MS, and gallic acid, 4-hydroxybenzoic acid, and benzoic acid were found to be the major phenolic compounds in the plants from both locations. Total phenolic- and flavonoid-content analyses of samples were carried out using spectrophotometry, and the stem extracts were found to be rich in phenolics. DPPH, ABTS, FRAP and CUPRAC assays were used to determine the antioxidant capacities of the extracts. A linear relation was observed between the phenolic contents of the extracts and their antioxidant activities, and the stem extracts of plants from both locations were found to have potent antioxidant capacity. The inhibitory activities of the extracts against acetylcholinesterase, butyrylcholinesterase and tyrosinase were determined using a 96-well microplate reader. The antibutyrylcholinesterase activity of the extracts was found to be the highest. The outcomes of these investigations were further explored, and the underlying structure of multivariate data was revealed using principal component analysis. This study presents the distribution of chemical constituents and biological activities of the different parts of A. pallens, and also contributes to further investigations of Allium species.http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000013E.pdfalliumphenolicsantioxidantanticholinesteraseantityrosinase |
| spellingShingle | Emir Ahmet Emir Ceren Chemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in Turkey Archives of Biological Sciences allium phenolics antioxidant anticholinesterase antityrosinase |
| title | Chemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in Turkey |
| title_full | Chemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in Turkey |
| title_fullStr | Chemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in Turkey |
| title_full_unstemmed | Chemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in Turkey |
| title_short | Chemical profiles and biological properties of methanol extracts of Allium pallens L. from different localities in Turkey |
| title_sort | chemical profiles and biological properties of methanol extracts of allium pallens l from different localities in turkey |
| topic | allium phenolics antioxidant anticholinesterase antityrosinase |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2020/0354-46642000013E.pdf |
| work_keys_str_mv | AT emirahmet chemicalprofilesandbiologicalpropertiesofmethanolextractsofalliumpallenslfromdifferentlocalitiesinturkey AT emirceren chemicalprofilesandbiologicalpropertiesofmethanolextractsofalliumpallenslfromdifferentlocalitiesinturkey |