Filter trapping protocol to detect aggregated proteins in human cell lines

Summary: The loss of protein homeostasis results in cytotoxic protein aggregates, a common hallmark of aging and neurological diseases. Here, we present an adjusted filter-trapping assay protocol to detect global aggregated proteins in human cell lines, via a high-sensitive protein staining method....

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Bibliographic Details
Main Authors: Jagat K. Chhipi-Shrestha, Minoru Yoshida, Shintaro Iwasaki
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004518