Generation and Testing of Fluorescent Adaptable Simple Theranostic (FAST) Proteins

This protocol provides a step-by-step method to create recombinant fluorescent fusion proteins that can be secreted from mammalian cell lines. This builds on many other recombinant protein and fluorescent protein techniques, but is among the first to harness fluorescent fusion proteins secreted dire...

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Bibliographic Details
Main Authors: Andrew Flies, Jocelyn Darby, Peter Murphy, Terry Pinfold, Amanda Patchett, Patrick Lennard
Format: Article
Language:English
Published: Bio-protocol LLC 2020-07-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=3696&type=0