Engineered cyclodextrin glucanotransferases from Bacillus sp. G‐825‐6 produce large‐ring cyclodextrins with high specificity

Engineered cyclodextrin glucanotransferases from Bacillus sp. G‐825‐6 produce large‐ring cyclodextrins with high specificity

Abstract Cyclodextrin glucanotransferases (CGTases) synthesize cyclic oligosaccharides (cyclodextrins, CD) from starch. A CGTase from Bacillus sp. G‐825‐6 was engineered by site‐directed mutagenesis at two positions by the construction of the variants Y183W, Y183R, D358R, Y183W/D358R, and Y183R/D358...

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Bibliographic Details
Main Authors: Christian Sonnendecker, Susanne Melzer, Wolfgang Zimmermann
Format: Article
Language:English
Published: Wiley 2019-06-01
Series:MicrobiologyOpen
Subjects:
Bacillus
cyclodextrin glucanotransferase
cyclodextrins
protein engineering
side‐directed mutagenesis
Online Access:https://doi.org/10.1002/mbo3.757

Internet

https://doi.org/10.1002/mbo3.757

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