Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> Larvae
Thermolabile grape berry proteins such as thaumatin-like proteins (TLPs) and chitinases (CHIs) promote haze formation in bottled wines if not properly fined. As a natural grapevine pest, the spotted-wing fly <i>Drosophila suzukii</i> is a promising source of peptidases that break down gr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/3/451 |
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| author | Wendell Albuquerque Parviz Ghezellou Kwang-Zin Lee Quintus Schneider Phillip Gross Tobias Kessel Bodunrin Omokungbe Bernhard Spengler Andreas Vilcinskas Holger Zorn Martin Gand |
| author_facet | Wendell Albuquerque Parviz Ghezellou Kwang-Zin Lee Quintus Schneider Phillip Gross Tobias Kessel Bodunrin Omokungbe Bernhard Spengler Andreas Vilcinskas Holger Zorn Martin Gand |
| author_sort | Wendell Albuquerque |
| collection | DOAJ |
| description | Thermolabile grape berry proteins such as thaumatin-like proteins (TLPs) and chitinases (CHIs) promote haze formation in bottled wines if not properly fined. As a natural grapevine pest, the spotted-wing fly <i>Drosophila suzukii</i> is a promising source of peptidases that break down grape berry proteins because the larvae develop and feed inside mature berries. Therefore, we produced recombinant TLP and CHI as model thermolabile wine haze proteins and applied a peptidomics strategy to investigate whether <i>D. suzukii</i> larval peptidases were able to digest them under acidic conditions (pH 3.5), which are typically found in winemaking practices. The activity of the novel peptidases was confirmed by mass spectrometry, and cleavage sites within the wine haze proteins were visualized in 3D protein models. The combination of recombinant haze proteins and peptidomics provides a valuable screening tool to identify optimal peptidases suitable for clarification processes in the winemaking industry. |
| first_indexed | 2024-03-11T06:52:47Z |
| format | Article |
| id | doaj.art-a2c401d1d88347f3989171e5c0a94e2c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T06:52:47Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-a2c401d1d88347f3989171e5c0a94e2c2023-11-17T09:51:29ZengMDPI AGBiomolecules2218-273X2023-02-0113345110.3390/biom13030451Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> LarvaeWendell Albuquerque0Parviz Ghezellou1Kwang-Zin Lee2Quintus Schneider3Phillip Gross4Tobias Kessel5Bodunrin Omokungbe6Bernhard Spengler7Andreas Vilcinskas8Holger Zorn9Martin Gand10Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyFraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392 Giessen, GermanyInstitute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyInstitute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyFraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392 Giessen, GermanyFraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392 Giessen, GermanyInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyFraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392 Giessen, GermanyInstitute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyInstitute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyThermolabile grape berry proteins such as thaumatin-like proteins (TLPs) and chitinases (CHIs) promote haze formation in bottled wines if not properly fined. As a natural grapevine pest, the spotted-wing fly <i>Drosophila suzukii</i> is a promising source of peptidases that break down grape berry proteins because the larvae develop and feed inside mature berries. Therefore, we produced recombinant TLP and CHI as model thermolabile wine haze proteins and applied a peptidomics strategy to investigate whether <i>D. suzukii</i> larval peptidases were able to digest them under acidic conditions (pH 3.5), which are typically found in winemaking practices. The activity of the novel peptidases was confirmed by mass spectrometry, and cleavage sites within the wine haze proteins were visualized in 3D protein models. The combination of recombinant haze proteins and peptidomics provides a valuable screening tool to identify optimal peptidases suitable for clarification processes in the winemaking industry.https://www.mdpi.com/2218-273X/13/3/451peptidasespeptidomics<i>Drosophila suzukii</i>thaumatin-like proteinschitinasesrecombinant proteins |
| spellingShingle | Wendell Albuquerque Parviz Ghezellou Kwang-Zin Lee Quintus Schneider Phillip Gross Tobias Kessel Bodunrin Omokungbe Bernhard Spengler Andreas Vilcinskas Holger Zorn Martin Gand Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> Larvae Biomolecules peptidases peptidomics <i>Drosophila suzukii</i> thaumatin-like proteins chitinases recombinant proteins |
| title | Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> Larvae |
| title_full | Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> Larvae |
| title_fullStr | Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> Larvae |
| title_full_unstemmed | Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> Larvae |
| title_short | Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from <i>Drosophila suzukii</i> Larvae |
| title_sort | peptidomics as a tool to assess the cleavage of wine haze proteins by peptidases from i drosophila suzukii i larvae |
| topic | peptidases peptidomics <i>Drosophila suzukii</i> thaumatin-like proteins chitinases recombinant proteins |
| url | https://www.mdpi.com/2218-273X/13/3/451 |
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