Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems

Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems

CRISPR/Cas-mediated genome editing in human pluripotent stem cells (hPSCs) offers unprecedented opportunities for developing in vitro disease modeling, drug screening and cell-based therapies. To efficiently deliver the CRISPR components, here we developed two all-in-one vectors containing Cas9/gRNA...

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Bibliographic Details
Main Authors: Yuqian Jiang, Rachel Catherine Hoenisch, Yun Chang, Xiaoping Bao, Craig E. Cameron, Xiaojun Lance Lian
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2022-08-01
Series:Bioactive Materials
Subjects:
CRISPR-Cas9
Genome editing
Cas13d
RNA editing
PiggyBac transposon
Human pluripotent stem cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2452199X22000615

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http://www.sciencedirect.com/science/article/pii/S2452199X22000615

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