Expression and Purification of the Thermus thermophilus Argonaute Protein
The Argonaute protein of Thermus thermophilus (TtAgo) has recently been studied in detail. For its in vitro characterization, TtAgo was purified after heterologous expression in Escherichia coli (E. coli). As TtAgo expression is toxic, a tightly controlled system was used for protein expression. The...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2014-10-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=1253&type=0 |