Cross-linked whole cells for the sucrose transfructosylation reaction in a continuous reactor
Fructooligosaccharides (FOS) are fructose oligomers beneficial to human health and nutrition for prebiotic sugars. Their production occurs by a transfructosylation reaction in sucrose molecules catalyzed by fructosyltransferase enzymes (FTase, E.C.2.4.1.9) adhered to microbial cells. The purpose of...
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| Format: | Article |
| Language: | English |
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Association of the Chemical Engineers of Serbia
2024-01-01
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| Series: | Chemical Industry and Chemical Engineering Quarterly |
| Subjects: | |
| Online Access: | https://doiserbia.nb.rs/img/doi/1451-9372/2024/1451-93722300015M.pdf |
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| author | Ribeiro Menossi Beatriz da Rin de Sandre Leandro Junior de Souza Dias Giancarlo da Cunha Abreu Xavier Michelle de Almeida Alex Fernando da Silva Elda Sabino Maiorano Alfredo Eduardo Firmani Perna Rafael Villalba Morales Sergio Andres |
| author_facet | Ribeiro Menossi Beatriz da Rin de Sandre Leandro Junior de Souza Dias Giancarlo da Cunha Abreu Xavier Michelle de Almeida Alex Fernando da Silva Elda Sabino Maiorano Alfredo Eduardo Firmani Perna Rafael Villalba Morales Sergio Andres |
| author_sort | Ribeiro Menossi Beatriz |
| collection | DOAJ |
| description | Fructooligosaccharides (FOS) are fructose oligomers beneficial to human health and nutrition for prebiotic sugars. Their production occurs by a transfructosylation reaction in sucrose molecules catalyzed by fructosyltransferase enzymes (FTase, E.C.2.4.1.9) adhered to microbial cells. The purpose of this work was to study the preparation, enzymatic activity, and stability of glutaraldehyde-crosslinked Aspergillus oryzae IPT-301 cells used as a biocatalyst for the transfructosylation reaction of sucrose in a packed bed reactor (PBR), aiming at FOS production. The highest transfructosylation activity (AT) was presented by the biocatalyst prepared by cross-linking at 200 rpm and 45 min. The highest AT in the PBR was obtained at 50 °C, with flow rates from 3 mL min-1 to 5 mL min-1 and sucrose concentrations of 473 g L-1 and 500 g L-1. The enzymatic kinetics was described using the Michaelis-Menten model. Finally, the biocatalyst showed constant AT of approximately 75 U g-1 and 300 U g-1 for 12 h of reaction in the PBR operating in continuous and discontinuous flow, respectively. These results demonstrate a high potential of glutaraldehyde-crosslinked A. oryzae IPT-301 cells as heterogeneous biocatalysts for the continuous production of FOS in PBR reactors. |
| first_indexed | 2024-04-24T11:31:10Z |
| format | Article |
| id | doaj.art-a31fe6f2f8fe40668b207d5bd6f446d2 |
| institution | Directory Open Access Journal |
| issn | 1451-9372 2217-7434 |
| language | English |
| last_indexed | 2024-04-24T11:31:10Z |
| publishDate | 2024-01-01 |
| publisher | Association of the Chemical Engineers of Serbia |
| record_format | Article |
| series | Chemical Industry and Chemical Engineering Quarterly |
| spelling | doaj.art-a31fe6f2f8fe40668b207d5bd6f446d22024-04-10T10:15:37ZengAssociation of the Chemical Engineers of SerbiaChemical Industry and Chemical Engineering Quarterly1451-93722217-74342024-01-013029911010.2298/CICEQ221220015M1451-93722300015MCross-linked whole cells for the sucrose transfructosylation reaction in a continuous reactorRibeiro Menossi Beatriz0da Rin de Sandre Leandro Junior1de Souza Dias Giancarlo2da Cunha Abreu Xavier Michelle3de Almeida Alex Fernando4da Silva Elda Sabino5Maiorano Alfredo Eduardo6Firmani Perna Rafael7Villalba Morales Sergio Andres8Institute of Science and Technology, Federal University of Alfenas, Poços de Caldas, BrazilInstitute of Science and Technology, Federal University of Alfenas, Poços de Caldas, BrazilInstitute of Science and Technology, Federal University of Alfenas, Poços de Caldas, BrazilBioprocess Engineering and Biotechnology Department, Federal University of Tocantins Gurupi, BrazilFood Engineering Department, Federal University of Tocantins, Palmas, BrazilIndustrial Biotechnology Laboratory, Institute for Technological Research University City, São Paulo, BrazilIndustrial Biotechnology Laboratory, Institute for Technological Research University City, São Paulo, BrazilInstitute of Science and Technology, Federal University of Alfenas, Poços de Caldas, BrazilBioprocess Engineering and Biotechnology Department, Federal University of Tocantins Gurupi, BrazilFructooligosaccharides (FOS) are fructose oligomers beneficial to human health and nutrition for prebiotic sugars. Their production occurs by a transfructosylation reaction in sucrose molecules catalyzed by fructosyltransferase enzymes (FTase, E.C.2.4.1.9) adhered to microbial cells. The purpose of this work was to study the preparation, enzymatic activity, and stability of glutaraldehyde-crosslinked Aspergillus oryzae IPT-301 cells used as a biocatalyst for the transfructosylation reaction of sucrose in a packed bed reactor (PBR), aiming at FOS production. The highest transfructosylation activity (AT) was presented by the biocatalyst prepared by cross-linking at 200 rpm and 45 min. The highest AT in the PBR was obtained at 50 °C, with flow rates from 3 mL min-1 to 5 mL min-1 and sucrose concentrations of 473 g L-1 and 500 g L-1. The enzymatic kinetics was described using the Michaelis-Menten model. Finally, the biocatalyst showed constant AT of approximately 75 U g-1 and 300 U g-1 for 12 h of reaction in the PBR operating in continuous and discontinuous flow, respectively. These results demonstrate a high potential of glutaraldehyde-crosslinked A. oryzae IPT-301 cells as heterogeneous biocatalysts for the continuous production of FOS in PBR reactors.https://doiserbia.nb.rs/img/doi/1451-9372/2024/1451-93722300015M.pdffructosyltransferasewhole cellsheterogeneous biocatalystspacked bed reactorsfructooligosaccharides |
| spellingShingle | Ribeiro Menossi Beatriz da Rin de Sandre Leandro Junior de Souza Dias Giancarlo da Cunha Abreu Xavier Michelle de Almeida Alex Fernando da Silva Elda Sabino Maiorano Alfredo Eduardo Firmani Perna Rafael Villalba Morales Sergio Andres Cross-linked whole cells for the sucrose transfructosylation reaction in a continuous reactor Chemical Industry and Chemical Engineering Quarterly fructosyltransferase whole cells heterogeneous biocatalysts packed bed reactors fructooligosaccharides |
| title | Cross-linked whole cells for the sucrose transfructosylation reaction in a continuous reactor |
| title_full | Cross-linked whole cells for the sucrose transfructosylation reaction in a continuous reactor |
| title_fullStr | Cross-linked whole cells for the sucrose transfructosylation reaction in a continuous reactor |
| title_full_unstemmed | Cross-linked whole cells for the sucrose transfructosylation reaction in a continuous reactor |
| title_short | Cross-linked whole cells for the sucrose transfructosylation reaction in a continuous reactor |
| title_sort | cross linked whole cells for the sucrose transfructosylation reaction in a continuous reactor |
| topic | fructosyltransferase whole cells heterogeneous biocatalysts packed bed reactors fructooligosaccharides |
| url | https://doiserbia.nb.rs/img/doi/1451-9372/2024/1451-93722300015M.pdf |
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