Rapid and Simple Amplification of Genomic DNA Sequences Flanking Transposon
A rapid and simple method to amplify genomic DNA sequences flanking mini-Tn5 transposon insertion was developed. This technique can be used to determine the location and orientation of the transposon insertion within genomic DNA of the bacteria. Based on the mini-Tn5Km1 transposon sequence, PCR prim...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Microbiology
2010-03-01
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| Series: | Microbiology Indonesia |
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| Online Access: | https://jurnal.permi.or.id/index.php/mionline/article/view/32 |