Therapeutic effects of Pheretima guillelmi on rat experimental model of alveolar bone resorption and mouse osteoblastic MC3T3-E1 cells(中药地龙提取液的促成骨作用及对实验性牙槽骨吸收疗效的研究)
探讨了中药地龙水提液和醇提液有无促进成骨细胞增殖、分化和基质矿化的作用及对大鼠牙槽骨吸收疗效进行了研究.制备地龙的水相及醇相提取液，以小鼠成骨细胞株MC3T3-E1作为体外药效的试验模型，分别通过MTT法.流式细胞术，碱性磷酸酶活性测定，骨钙素检测和Von kossa基质矿化染色法观察上述药物的促细胞增殖、分化、基质矿化作用.以实验牙位龈正中注射脂多糖建立的SD大鼠实验性牙槽骨吸收作为体内药效试验模型，通过组织切片形态学骨密度观察和抗酒石酸酸性磷酸酶染色评价上述药物对大鼠实验性牙槽骨吸收的疗效.结果发现：1 mg • L-1水提液，0.01 mg • L-1和1 mg • L-1醇提液能显著促...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Zhejiang University Press
2008-11-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2008.06.021 |