Targeting the 16S rRNA Gene by Reverse Complement PCR Next-Generation Sequencing: Specific and Sensitive Detection and Identification of Microbes Directly in Clinical Samples

Targeting the 16S rRNA Gene by Reverse Complement PCR Next-Generation Sequencing: Specific and Sensitive Detection and Identification of Microbes Directly in Clinical Samples

ABSTRACT The detection and accurate identification of bacterial species in clinical samples are crucial for diagnosis and appropriate antibiotic treatment. To date, sequencing of the 16S rRNA gene has been widely used as a complementary molecular approach when identification by culture fails. The ac...

Full description

Bibliographic Details
Main Authors: Simone J. C. F. M. Moorlag, Jordy P. M. Coolen, Bart van den Bosch, Elisabeth Hui-Mei Jin, Jochem B. Buil, Heiman F. L. Wertheim, Willem J. G. Melchers
Format: Article
Language:English
Published: American Society for Microbiology 2023-06-01
Series:Microbiology Spectrum
Subjects:
16S rRNA
reverse complement PCR
RC-PCR
bacterial identification
NGS
molecular diagnostics
Online Access:https://journals.asm.org/doi/10.1128/spectrum.04483-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.04483-22

Similar Items