Strategies for optimizing CITE-seq for human islets and other tissues

Strategies for optimizing CITE-seq for human islets and other tissues

Defining the immunological landscape of human tissue is an important area of research, but challenges include the impact of tissue disaggregation on cell phenotypes and the low abundance of immune cells in many tissues. Here, we describe methods to troubleshoot and standardize Cellular Indexing of T...

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Bibliographic Details
Main Authors: Sarah J. Colpitts, Matthew A. Budd, Mahdis Monajemi, Kyle T. Reid, Julia M. Murphy, Sabine Ivison, C. Bruce Verchere, Megan K. Levings, Sarah Q. Crome
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Immunology
Subjects:
CITE-seq
tissue immunity
flow cytometry
pancreas
single cell RNA seq
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2023.1107582/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2023.1107582/full

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