Fluorescent pulse-chase labeling to monitor long-term mitochondrial degradation in primary hippocampal neurons

Fluorescent pulse-chase labeling to monitor long-term mitochondrial degradation in primary hippocampal neurons

Summary: The accumulation of dysfunctional mitochondria is a hallmark of neurodegenerative diseases, yet the dynamics of mitochondrial turnover in neurons are unclear. Here, we describe a protocol to monitor the degradation of spectrally distinct, “aged” mitochondrial populations. We describe the pr...

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Bibliographic Details
Main Authors: Jordan R. Schneider, Chantell S. Evans
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell-based Assays
Metabolism
Microscopy
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200702X

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http://www.sciencedirect.com/science/article/pii/S266616672200702X

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