Enhanced Efficiency of flySAM by Optimization of sgRNA Parameters in Drosophila

Enhanced Efficiency of flySAM by Optimization of sgRNA Parameters in Drosophila

The flySAM/CRISPRa system has recently emerged as a powerful tool for gain-of-function studies in Drosophila melanogaster. This system includes Gal4/UAS-driven dCas9 activators and U6 promoter-controlled sgRNA. Having established dCas9 activators superior to other combinations, to further enhance th...

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Bibliographic Details
Main Authors: Decai Mao, Yu Jia, Ping Peng, Da Shen, Xingjie Ren, Ruibao Zhu, Yuhao Qiu, Yuting Han, Jinchao Yu, Qinyun Che, Yutong Li, Xinyi Lu, Lu-Ping Liu, Zhao Wang, Qingfei Liu, Jin Sun, Jian-Quan Ni
Format: Article
Language:English
Published: Oxford University Press 2020-12-01
Series:G3: Genes, Genomes, Genetics
Subjects:
flysam
sgrna
position effect
gc content
dna strand
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.120.401614

Internet

http://g3journal.org/lookup/doi/10.1534/g3.120.401614

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