A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study
An LC-MS/MS method has been developed for the determination of efavirenz (EFZ) in human plasma using hydrochlorothiazide as internal standard (I.S.). An ESI negative mode with multiple reaction-monitoring was used monitoring the transitions m/z 313.88→69.24 (EFZ) and 296.02→204.76 (I.S.). Samples we...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2011-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000600007&lng=en&tlng=en |
| _version_ | 1819112546419867648 |
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| author | Danilo Cesar Galindo Bedor Jose Homero de Souza Filho Virna Ligiane Souza Ramos Talita Mota Gonçalves Carlos Eduardo Miranda de Sousa Davi Pereira de Santana |
| author_facet | Danilo Cesar Galindo Bedor Jose Homero de Souza Filho Virna Ligiane Souza Ramos Talita Mota Gonçalves Carlos Eduardo Miranda de Sousa Davi Pereira de Santana |
| author_sort | Danilo Cesar Galindo Bedor |
| collection | DOAJ |
| description | An LC-MS/MS method has been developed for the determination of efavirenz (EFZ) in human plasma using hydrochlorothiazide as internal standard (I.S.). An ESI negative mode with multiple reaction-monitoring was used monitoring the transitions m/z 313.88→69.24 (EFZ) and 296.02→204.76 (I.S.). Samples were extracted using liquid-liquid extraction. The total run time was 2.0 min. The separation was achieved with HPLC-RP using a monolithic column. The assay was linear in the concentration range of 100 - 5000 ng mL-1. The mean recovery was 83%. Intra- and inter-day precision were < 9.5% and < 8.9%, respectively and accuracy was in the range ± 8.33%. The method was successfully applied to a bioequivalence study. |
| first_indexed | 2024-12-22T04:15:14Z |
| format | Article |
| id | doaj.art-a3a7b7309bf549eabcd306f2b36783db |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-12-22T04:15:14Z |
| publishDate | 2011-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-a3a7b7309bf549eabcd306f2b36783db2022-12-21T18:39:25ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642011-01-0134695095510.1590/S0100-40422011000600007S0100-40422011000600007A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence studyDanilo Cesar Galindo Bedor0Jose Homero de Souza Filho1Virna Ligiane Souza Ramos2Talita Mota Gonçalves3Carlos Eduardo Miranda de Sousa4Davi Pereira de Santana5Universidade Federal de PernambucoUniversidade Federal de PernambucoUniversidade Federal de PernambucoUniversidade Federal de PernambucoUniversidade Federal de PernambucoUniversidade Federal de PernambucoAn LC-MS/MS method has been developed for the determination of efavirenz (EFZ) in human plasma using hydrochlorothiazide as internal standard (I.S.). An ESI negative mode with multiple reaction-monitoring was used monitoring the transitions m/z 313.88→69.24 (EFZ) and 296.02→204.76 (I.S.). Samples were extracted using liquid-liquid extraction. The total run time was 2.0 min. The separation was achieved with HPLC-RP using a monolithic column. The assay was linear in the concentration range of 100 - 5000 ng mL-1. The mean recovery was 83%. Intra- and inter-day precision were < 9.5% and < 8.9%, respectively and accuracy was in the range ± 8.33%. The method was successfully applied to a bioequivalence study.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000600007&lng=en&tlng=enefavirenzbioequivalenceLC-MS/MS |
| spellingShingle | Danilo Cesar Galindo Bedor Jose Homero de Souza Filho Virna Ligiane Souza Ramos Talita Mota Gonçalves Carlos Eduardo Miranda de Sousa Davi Pereira de Santana A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study Química Nova efavirenz bioequivalence LC-MS/MS |
| title | A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study |
| title_full | A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study |
| title_fullStr | A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study |
| title_full_unstemmed | A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study |
| title_short | A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study |
| title_sort | sensitive and robust lc ms ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma application to a bioequivalence study |
| topic | efavirenz bioequivalence LC-MS/MS |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422011000600007&lng=en&tlng=en |
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