Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue
Summary: Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effecti...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722003264 |
| _version_ | 1818552766866391040 |
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| author | Natalia Mendelev Michel Zamojski Mary Anne S. Amper Wan Sze Cheng Hanna Pincas Venugopalan D. Nair Elena Zaslavsky Stuart C. Sealfon Frederique Ruf-Zamojski |
| author_facet | Natalia Mendelev Michel Zamojski Mary Anne S. Amper Wan Sze Cheng Hanna Pincas Venugopalan D. Nair Elena Zaslavsky Stuart C. Sealfon Frederique Ruf-Zamojski |
| author_sort | Natalia Mendelev |
| collection | DOAJ |
| description | Summary: Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline.For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-12-12T09:17:28Z |
| format | Article |
| id | doaj.art-a3a9214cfbe7450d9f5f76742d262703 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-12T09:17:28Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-a3a9214cfbe7450d9f5f76742d2627032022-12-22T00:29:21ZengElsevierSTAR Protocols2666-16672022-06-0132101446Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissueNatalia Mendelev0Michel Zamojski1Mary Anne S. Amper2Wan Sze Cheng3Hanna Pincas4Venugopalan D. Nair5Elena Zaslavsky6Stuart C. Sealfon7Frederique Ruf-Zamojski8Department of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USADepartment of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USADepartment of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USADepartment of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USADepartment of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USADepartment of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USADepartment of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USADepartment of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USA; Corresponding authorDepartment of Neurology, Center for Advanced Research on Diagnostic Assays, Icahn School of Medicine at Mount Sinai (ISMMS), New York, NY, USA; Corresponding authorSummary: Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline.For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003264Cell BiologySingle CellHealth SciencesGenomicsSequencingRNAseq |
| spellingShingle | Natalia Mendelev Michel Zamojski Mary Anne S. Amper Wan Sze Cheng Hanna Pincas Venugopalan D. Nair Elena Zaslavsky Stuart C. Sealfon Frederique Ruf-Zamojski Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue STAR Protocols Cell Biology Single Cell Health Sciences Genomics Sequencing RNAseq |
| title | Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue |
| title_full | Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue |
| title_fullStr | Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue |
| title_full_unstemmed | Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue |
| title_short | Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue |
| title_sort | multi omics profiling of single nuclei from frozen archived postmortem human pituitary tissue |
| topic | Cell Biology Single Cell Health Sciences Genomics Sequencing RNAseq |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722003264 |
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