Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate

Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system by electroporation (TAKE), aids in the production of genome-edited animals by introducing nucleases into intact embryos using electroporation...

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Bibliographic Details
Main Authors: Yui WAKE, Christopher A. VAKULSKAS, Steve E. GLENN, Takehito KANEKO
Format: Article
Language:English
Published: The Society for Reproduction and Development 2022-07-01
Series:The Journal of Reproduction and Development
Subjects:
Online Access:https://www.jstage.jst.go.jp/article/jrd/68/5/68_2022-067/_pdf/-char/en