Amount of Cas9 protein introduced into mouse embryos via electroporation affects the genome-editing rate
Genetically engineered animals can be produced quickly using genome editing technology. A new electroporation technique, technique for animal knockout system by electroporation (TAKE), aids in the production of genome-edited animals by introducing nucleases into intact embryos using electroporation...
Main Authors: | Yui WAKE, Christopher A. VAKULSKAS, Steve E. GLENN, Takehito KANEKO |
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Format: | Article |
Language: | English |
Published: |
The Society for Reproduction and Development
2022-07-01
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Series: | The Journal of Reproduction and Development |
Subjects: | |
Online Access: | https://www.jstage.jst.go.jp/article/jrd/68/5/68_2022-067/_pdf/-char/en |
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