Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond
Abstract More than 40 years ago, Howard Green's laboratory developed a method for long‐term expansion of primary human epidermal keratinocytes by co‐culture with 3T3 mouse embryonic fibroblasts. This was a breakthrough for in vitro cultivation of cells from human skin and later for other epithe...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2017-12-01
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| Series: | EMBO Molecular Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.15252/emmm.201708213 |
| _version_ | 1827059780868374528 |
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| author | Robert E Hynds Paola Bonfanti Sam M Janes |
| author_facet | Robert E Hynds Paola Bonfanti Sam M Janes |
| author_sort | Robert E Hynds |
| collection | DOAJ |
| description | Abstract More than 40 years ago, Howard Green's laboratory developed a method for long‐term expansion of primary human epidermal keratinocytes by co‐culture with 3T3 mouse embryonic fibroblasts. This was a breakthrough for in vitro cultivation of cells from human skin and later for other epithelia: it led to the first stem cell therapy using cultured cells and has vastly increased our understanding of epithelial stem cell biology. In recent years, new methods to expand epithelial cells as three‐dimensional organoids have provided novel means to investigate the functions of these cells in health and disease. Here, we outline the history of stratified epithelial stem cell culture and the application of cultured epithelial cells in clinical therapies. We further discuss the derivation of organoids from other types of epithelia and the challenges that remain for the translation of novel stem cell therapies toward clinical use. |
| first_indexed | 2024-03-07T17:08:02Z |
| format | Article |
| id | doaj.art-a3df8cc72dc746c38dfea7f62971a93e |
| institution | Directory Open Access Journal |
| issn | 1757-4676 1757-4684 |
| language | English |
| last_indexed | 2025-02-18T18:46:54Z |
| publishDate | 2017-12-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | EMBO Molecular Medicine |
| spelling | doaj.art-a3df8cc72dc746c38dfea7f62971a93e2024-10-20T11:39:50ZengSpringer NatureEMBO Molecular Medicine1757-46761757-46842017-12-0110213915010.15252/emmm.201708213Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyondRobert E Hynds0Paola Bonfanti1Sam M Janes2Lungs for Living Research Centre, UCL Respiratory, University College LondonThe Francis Crick InstituteLungs for Living Research Centre, UCL Respiratory, University College LondonAbstract More than 40 years ago, Howard Green's laboratory developed a method for long‐term expansion of primary human epidermal keratinocytes by co‐culture with 3T3 mouse embryonic fibroblasts. This was a breakthrough for in vitro cultivation of cells from human skin and later for other epithelia: it led to the first stem cell therapy using cultured cells and has vastly increased our understanding of epithelial stem cell biology. In recent years, new methods to expand epithelial cells as three‐dimensional organoids have provided novel means to investigate the functions of these cells in health and disease. Here, we outline the history of stratified epithelial stem cell culture and the application of cultured epithelial cells in clinical therapies. We further discuss the derivation of organoids from other types of epithelia and the challenges that remain for the translation of novel stem cell therapies toward clinical use.https://doi.org/10.15252/emmm.2017082133T3 cellsadult stem cellscell cultureepithelial cellsorganoids |
| spellingShingle | Robert E Hynds Paola Bonfanti Sam M Janes Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond EMBO Molecular Medicine 3T3 cells adult stem cells cell culture epithelial cells organoids |
| title | Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond |
| title_full | Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond |
| title_fullStr | Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond |
| title_full_unstemmed | Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond |
| title_short | Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond |
| title_sort | regenerating human epithelia with cultured stem cells feeder cells organoids and beyond |
| topic | 3T3 cells adult stem cells cell culture epithelial cells organoids |
| url | https://doi.org/10.15252/emmm.201708213 |
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