Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases
Bacterial extracellular proteases from six strains of marine bacteria and seven strains of terrestrial bacteria were prepared through fermentation. Proteases were analyzed through substrate immersing zymography and used to hydrolyze the collagen and muscle proteins from a salmon skin byproduct, resp...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-01-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/15/1/4 |
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| author | Ribang Wu Leilei Chen Dan Liu Jiafeng Huang Jiang Zhang Xiao Xiao Ming Lei Yuelin Chen Hailun He |
| author_facet | Ribang Wu Leilei Chen Dan Liu Jiafeng Huang Jiang Zhang Xiao Xiao Ming Lei Yuelin Chen Hailun He |
| author_sort | Ribang Wu |
| collection | DOAJ |
| description | Bacterial extracellular proteases from six strains of marine bacteria and seven strains of terrestrial bacteria were prepared through fermentation. Proteases were analyzed through substrate immersing zymography and used to hydrolyze the collagen and muscle proteins from a salmon skin byproduct, respectively. Collagen could be degraded much more easily than muscle protein, but it commonly showed weaker antioxidant capability. The hydrolysate of muscle proteins was prepared with crude enzymes from Pseudoalteromonas sp. SQN1 displayed the strongest activity of antioxidant in DPPH and hydroxyl radical scavenging assays (74.06% ± 1.14% and 69.71% ± 1.97%), but did not perform well in Fe2+ chelating assay. The antioxidant fractions were purified through ultrafiltration, cation exchange chromatography, and size exclusion chromatography gradually, and the final purified fraction U2-S2-I displayed strong activity of antioxidant in DPPH, hydroxyl radical scavenging assays (IC50 = 0.263 ± 0.018 mg/mL and 0.512 ± 0.055 mg/mL), and oxygen radical absorption capability assay (1.960 ± 0.381 mmol·TE/g). The final purified fraction U2-S2-I possessed the capability to protect plasmid DNA against the damage of hydroxyl radical and its effect was similar to that of the original hydrolysis product. It indicated that U2-S2-I might be the major active fraction of the hydrolysate. This study proved that bacterial extracellular proteases could be utilized in hydrolysis of a salmon byproduct. Compared with collagen, muscle proteins was an ideal material used as an enzymatic substrate to prepare antioxidant peptides. |
| first_indexed | 2024-04-11T20:44:35Z |
| format | Article |
| id | doaj.art-a3f2ff7bb7874a6694daecd39d2f8701 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T20:44:35Z |
| publishDate | 2017-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a3f2ff7bb7874a6694daecd39d2f87012022-12-22T04:04:05ZengMDPI AGMarine Drugs1660-33972017-01-01151410.3390/md15010004md15010004Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular ProteasesRibang Wu0Leilei Chen1Dan Liu2Jiafeng Huang3Jiang Zhang4Xiao Xiao5Ming Lei6Yuelin Chen7Hailun He8School of Life Science, State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaInstitute of Agro-Food Science and Technology & Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaSchool of Life Science, State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaSchool of Life Science, State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaSchool of Life Science, State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaSchool of Life Science, State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaSchool of Life Science, State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaSchool of Life Science, State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaSchool of Life Science, State Key Laboratory of Medical Genetics, Central South University, Changsha 410013, ChinaBacterial extracellular proteases from six strains of marine bacteria and seven strains of terrestrial bacteria were prepared through fermentation. Proteases were analyzed through substrate immersing zymography and used to hydrolyze the collagen and muscle proteins from a salmon skin byproduct, respectively. Collagen could be degraded much more easily than muscle protein, but it commonly showed weaker antioxidant capability. The hydrolysate of muscle proteins was prepared with crude enzymes from Pseudoalteromonas sp. SQN1 displayed the strongest activity of antioxidant in DPPH and hydroxyl radical scavenging assays (74.06% ± 1.14% and 69.71% ± 1.97%), but did not perform well in Fe2+ chelating assay. The antioxidant fractions were purified through ultrafiltration, cation exchange chromatography, and size exclusion chromatography gradually, and the final purified fraction U2-S2-I displayed strong activity of antioxidant in DPPH, hydroxyl radical scavenging assays (IC50 = 0.263 ± 0.018 mg/mL and 0.512 ± 0.055 mg/mL), and oxygen radical absorption capability assay (1.960 ± 0.381 mmol·TE/g). The final purified fraction U2-S2-I possessed the capability to protect plasmid DNA against the damage of hydroxyl radical and its effect was similar to that of the original hydrolysis product. It indicated that U2-S2-I might be the major active fraction of the hydrolysate. This study proved that bacterial extracellular proteases could be utilized in hydrolysis of a salmon byproduct. Compared with collagen, muscle proteins was an ideal material used as an enzymatic substrate to prepare antioxidant peptides.http://www.mdpi.com/1660-3397/15/1/4bacterial extracellular proteasesantioxidant peptideenzymatic hydrolysispeptide purificationevaluation of antioxidant activity |
| spellingShingle | Ribang Wu Leilei Chen Dan Liu Jiafeng Huang Jiang Zhang Xiao Xiao Ming Lei Yuelin Chen Hailun He Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases Marine Drugs bacterial extracellular proteases antioxidant peptide enzymatic hydrolysis peptide purification evaluation of antioxidant activity |
| title | Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases |
| title_full | Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases |
| title_fullStr | Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases |
| title_full_unstemmed | Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases |
| title_short | Preparation of Antioxidant Peptides from Salmon Byproducts with Bacterial Extracellular Proteases |
| title_sort | preparation of antioxidant peptides from salmon byproducts with bacterial extracellular proteases |
| topic | bacterial extracellular proteases antioxidant peptide enzymatic hydrolysis peptide purification evaluation of antioxidant activity |
| url | http://www.mdpi.com/1660-3397/15/1/4 |
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