Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO
Summary: Visualization of mRNA molecules in single cells has revealed their core mechanisms as well as sources of cell-to-cell and spatiotemporal heterogeneity. Here, we describe a protocol to label, visualize, and quantify mRNA molecules by time-lapse imaging with the capability of resolving mRNA m...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-09-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672200510X |