Label and quantify mRNA molecules in live cell experiments using SunRISER and dNEMO

Summary: Visualization of mRNA molecules in single cells has revealed their core mechanisms as well as sources of cell-to-cell and spatiotemporal heterogeneity. Here, we describe a protocol to label, visualize, and quantify mRNA molecules by time-lapse imaging with the capability of resolving mRNA m...

Full description

Bibliographic Details
Main Authors: Yue Guo, Gabriel J. Kowalczyk, Robin E.C. Lee
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200510X