Selection and Validation of Reliable Reference Genes for qRT-PCR Normalization of <i>Bursaphelenchus xylophilus</i> from Different Temperature Conditions and Developmental Stages

Selection and Validation of Reliable Reference Genes for qRT-PCR Normalization of <i>Bursaphelenchus xylophilus</i> from Different Temperature Conditions and Developmental Stages

Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a powerful technique for studying gene expression. The key to quantitative accuracy depends on the stability of the reference genes used for data normalization under different experimental conditions. Pine wood nematode (PWN,...

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Bibliographic Details
Main Authors: Yajing Wu, Jiao Zhou, Yaning Liu, Yutong Gu, Hongxia Zhang, Faheem Ahmad, Guochang Wang, Lili Ren
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Applied Sciences
Subjects:
<i>Bursaphelenchus xylophilus</i>
reference gene
qRT-PCR
normalization
Online Access:https://www.mdpi.com/2076-3417/12/6/2880

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https://www.mdpi.com/2076-3417/12/6/2880

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