In vivo and in vitro Cleavage of Glucoamylase-TNFα Fusion Protein Secreted from Aspergillus niger

In vivo and in vitro Cleavage of Glucoamylase-TNFα Fusion Protein Secreted from Aspergillus niger

The most common expression strategy for secreting heterologous proteins from filamentous fungus Aspergillus niger is based on fusion with glucoamylase gene which contains cleavage site for kexin protease (KEX2). However, secretion of recombinant proteins in the form of a fusion-protein without a hos...

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Bibliographic Details
Main Authors: Monika Svetina, Vladka Gaberc-Porekar, Viktor Menart, Radovan Komel, Nada Kraševec
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2003-01-01
Series:Food Technology and Biotechnology
Subjects:
filamentous fungi
Aspergillus niger
heterologous protein secretion
human tumor necrosis factor α (TNFα) KEX2 cleavage site
enterokinase cleavage site
His-tag
Online Access:http://hrcak.srce.hr/file/185064

Internet

http://hrcak.srce.hr/file/185064

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